LBA4404 Chemically Competent Agrobacterium are useful for transgenic operations of tomatoes, tobacco and other plants.
Description
Intact Genomics LBA4404 Chemically Competent Agrobacterium cells are optimized for the highest transformation efficiencies which is ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. The LBA4404 strain is useful for transgenic operations of tomatoes, tobacco and other plants. LBA4404 contains a rifampicin resistance gene (rif). LBA4404 strain also contains a octoprine-type Ti plasmid pAL4404 without self-transport function, which contains the vir gene.
Specifications
Reagents Included
- LBA4404 Chemically Competent Agrobacterium
- DNA (pCAMBIA1391z, 500 pg/µl)
- Recovery medium
Note: Liquid nitrogen is required.
Storage
Quality Control
General Guidelines
Follow these guidelines when using LBA4404 Chemically Competent Agrobacterium cells:
- Handle competent cells gently as they are highly sensitive to changes in temperature or mechanical lysis caused by pipetting.
- Thaw competent cells on ice, and transform cells immediately following thawing. After adding DNA, mix by tapping the tube gently. Do not mix cells by pipetting or vortexing.
Calculation of Transformation Efficiency
Transformation Efficiency (TE) is defined as the number of colony forming units (cfu) produced by transforming 1µg of plasmid into a given volume of competent cells.
TE = Colonies/µg/Plated
Transform 1 µl of (500 pg/µl) pCAMBIA1391z control plasmid into 50 µl of cells, add 950 µl of Recovery Medium. Recover for 3 hours and plate 100 µl. Count the colonies on the plate in two days. If you count 5 colonies, the TE is calculated as follows:
Colonies = 5
µg of DNA = 0.0005
Dilution = 100/1000 = 0.1
TE = 5/.0005/.1 = 1×105
Please note, all agrobacterial strains are not well studied for antibiotic resistance and there are many agrobacterial strains. Therefore, it is the customer’s responsibility to make sure his/her vectors are compatible with the Agrobacterial strains if he/she uses an alternate antibiotic selection than kanamycin-selection.