pMEV2HA-MEKK1-KD

Mouse MEKK1 Xpress Clones MAPK pathways play a key role in mediating cell growth and apoptosis. Among the components of the MAPK family, mitogen-activated protein kinase kinase kinase (MEKK1) is a 196-kDa serine-threonine kinase that was first cloned from a cDNA library derived from NIH 3T3 cells by virtue of its homology to Ste11 and Byr2. MEKK1 is activated in response to cytokines and various stresses, with GSK3 beta as one of the direct activators. MEKK1 preferentially activates the JNK pathway and also influences the activity of the ERK pathway, while it has little or no effect on the p38-MAPK pathways. MEKK1 has been reported to be involved in cardiac hypertrophy, mitochondrial permeability and apoptosis, focal adhesion and cell migration, activating C/EBP-beta-dependent gene expression in response to IFN-gamma.MEKK1-KD is the c-terminal protein kinase domain of mouse MEKK1, consisting of amino acid residues 375-672. It is constitutively active and has been used to activate MAPK pathways in transfection assays. Conserved in all protein kinases, lysine 452 in MEKK1 is catalytically essential. Changing it to a methionine, as is the case in MEKK1-K452M mutant, results in a mutant protein essentially lacking kinase activity. - 20 ug